Publications

* Contributed equally; # Correspondence

2025

1. Yan Y,  Zhou XE, Thomas SL, Liu M, Lai GQ, Worden EJ, Jones PA#, Xu TH#. Molecular Mechanisms of DNMT3A-3L-Mediated de novo DNA Methylation on Chromatin. bioRxiv. 2025. doi: 10.1101/2025.06.10.658647

2024

2. Xuan H, Xu L, Li K, Xuan F, Xu TH, Wen H, Shi X. Hotspot cancer mutation impairs KAT8-mediated nucleosomal histone acetylation. J Mol Biol 436. 2024. doi: 10.1016/j.jmb.2023.168413

2023

3. Thomas SL*, Xu TH*, Carpenter BL, Pierce SE, Dickson BM, Liu M, Liang G, Jones PA. DNA strand asymmetry generated by CpG hemimethylation has opposing effects on CTCF binding. Nucleic Acids Res. 2023. doi: 10.1093/nar/gkad293

2021

4. Yan Y, Mukherjee S, Harikumar KG, Strutzenberg TS, Zhou XE, Suino-Powell K, Xu TH, Sheldon RD, Lamp J, Brunzelle JS, Radziwon K, Ellis A, Novick SJ, Vega IE, Jones RG, Miller LJ, Xu HE, Griffin PR, Kossiakoff AA, Melcher K. Structure of an AMPK complex in an inactive, ATP-bound state. Science 373. 2021. doi: 10.1126/science.abe7565 

5. Yan Y, Mukherjee S, Harikumar KG, Strutzenberg TS, Zhou XE, Suino-Powell K, Xu TH, Sheldon RD, Lamp J, Brunzelle JS, Radziwon K, Ellis A, Novick SJ, Vega IE, Jones RG, Miller LJ, Xu HE, Griffin PR, Kossiakoff AA, Melcher K, editors. Structure of the AMPK complex in its inactive ATP-bound state. PROTEIN SCI. 2021.

6. Zhuang Y, Xu P, Mao C, Wang L, Krumm B, Zhou XE, Huang S, Liu H, Cheng X, Huang XP, Shen DD, Xu TH, Liu YF, Wang Y, Guo J, Jiang Y, Jiang H, Melcher K, Roth BL, Zhang Y, Zhang C, Xu HE. Structural insights into the human D1 and D2 dopamine receptor signaling complexes. Cell 184. 2021. doi: 10.1016/j.cell.2021.01.027

2020

7. Xu TH, Liu M, Zhou XE, Liang G, Zhao G, Xu HE, Melcher K, Jones PA. Structure of nucleosome-bound DNA methyltransferases DNMT3A and DNMT3B. Nature 586, 2020. doi: 10.1038/s41586-020-2747-1 

8. Xu TH, Liu M, Zhou XE, Liang G, Xu HE, Melcher K, Jones PA, editors. The nucleosomal acidic patch helps anchor of de novo DNA methyltransferase DNMT3A2/3B3 complex. Cancer Research. 2020. doi:10.1158/1538-7445.AM2020-1078

9. Xing C*, Zhuang Y*, Xu TH*, Feng Z*, Zhou XE, Chen M, Wang L, Meng X, Xue Y, Wang J, Liu H, McGuire TF, Zhao G, Melcher K, Zhang C, Xu HE, Xie XQ. Cryo-EM Structure of the Human Cannabinoid Receptor CB2-Gi Signaling Complex. Cell 180. 2020. doi: 10.1016/j.cell.2020.01.007 

10. Zhuang Y, Liu H, Zhou XE, Kumar Verma R, de Waal PW, Jang W, Xu TH, Wang L, Meng X, Zhao G, Kang Y, Melcher K, Fan H, Lambert NA, Xu HE, Zhang C. Structure of formylpeptide receptor 2-Gi complex reveals insights into ligand recognition and signaling. Nat Commun 11. 2020. doi: 10.1038/s41467-020-14728-9 

2018

11. Yang S, Wu Y*, Xu TH*, de Waal PW*, He Y, Pu M, Chen Y, DeBruine ZJ, Zhang B, Zaidi SA, Popov P, Guo Y, Han GW, Lu Y, Suino-Powell K, Dong S, Harikumar KG, Miller LJ, Katritch V, Xu HE, Shui W, Stevens RC, Melcher K, Zhao S, Xu F. Crystal structure of the Frizzled 4 receptor in a ligand-free state. Nature 560. 2018. doi: 10.1038/s41586-018-0447-x

2017

12. Yan Y*, Xu TH*, Harikumar KG, Miller LJ, Melcher K, Xu HE. Dimerization of the transmembrane domain of amyloid precursor protein is determined by residues around the gamma-secretase cleavage sites. J Biol Chem 292. 2017. doi: 10.1074/jbc.M117.789669

13. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. Amyloid beta: structure, biology and structure-based therapeutic development. Acta pharmacol Sin. 38. 2017. doi: 10.1038/aps.2017.28

14. Ma H, Duan J, Ke J, He Y, Gu X, Xu TH, Yu H, Wang Y, Brunzelle JS, Jiang Y, Rothbart SB, Xu HE, Li J, Melcher K. A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex. Sci Adv 3. 2017. doi: 10.1126/sciadv.1601217

15. Yan Y*, Xu TH*, Melcher K, Xu HE. Defining the minimum substrate and charge recognition model of gamma-secretase. Acta pharmacol Sin 38. 2017. doi: 10.1038/aps.2017.35

16. Yan Y, Xu TH, Harikumar KG, Miller LJ, Melcher K, Xu HE. Detection of membrane protein interactions by cell-based Tango assays. Bio Protoc 7. 2017. doi: 10.21769/BioProtoc.2903 

17. Harikumar KG, Yan Y, Xu TH, Melcher K, Xu HE, Miller LJ. Bioluminescence resonance energy transfer (BRET) assay for determination of molecular interactions in living cells. Bio Protoc 7. 2017. doi: 10.21769/BioProtoc.2904 

18. Xu TH, Yan Y, Harikumar KG, Miller LJ, Melcher K, Xu HE. Streptavidin bead pulldown assay to determine protein homooligomerization. Bio Protoc 7. 2017. doi: 10.21769/BioProtoc.2901 

19. Xu TH, Yan Y, Harikumar KG, Miller LJ, Melcher K, Xu HE. gamma-secretase epsilon-cleavage assay. Bio Protoc 7. 2017. doi: 10.21769/BioProtoc.2900 

2016

20. Xu TH, Yan Y, Kang Y, Jiang Y, Melcher K, Xu HE. Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to gamma-secretase cleavage and the Abeta42/Abeta40 ratio. Cell Discov 2. 2016. doi: 10.1038/celldisc.2016.26

2013

21. Zhao LH, Zhou XE, Wu ZS, Yi W, Xu Y, Li S, Xu TH, Liu Y, Chen RZ, Kovach A, Kang Y, Hou L, He Y, Xie C, Song W, Zhong D, Xu Y, Wang Y, Li J, Zhang C, Melcher K, Xu HE. Crystal structures of two phytohormone signal-transducing alpha/beta hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14. Cell Res 23. 2013. doi: 10.1038/cr.2013.19